Comparasion of the effectiveness of anchor proteins ScAGα1p, KpCW51p, KpCW61p for surface display in yeast <i>Komagataella phaffii</i>

BACKGROUND: Yeast display is an effective technology for exposure target proteins to the cell surface by fusing them with wall proteins. This technique, among other things, makes it possible obtain vaccine preparations based on yeast exposing antigen their surface. Finding and selecting that allow of cells urgent task.
 AIM: The aim this work was evaluate efficiency ScAG1p, KpCW51p, KpCW61p displaying reporter protein Komagataella phaffii surface, including study several variants ScAG1 gene coding sequence.
 MATERIALS AND METHODS: studied sequences were cloned under control AOX1 promoter in same reading frame as eGFP integrated into genome K. Phaffii strain X-33.
 RESULTS: Cytoimmunochemical analysis confocal microscopy strains conditions induction made identify most anchor protein. best demonstrated sequence without native 3' non-coding region.
 CONCLUSIONS: plasmids obtained will make a effectively proteins, antigens, its which can be used preparation.